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Elektroporatie van functionele bacteriële effectoren in zoogdiercellen
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1Biological Sciences Division,Pacific Northwest National Laboratory, 2Environmental Molecular Science Laboratory,Pacific Northwest National Laboratory, 3Structural Proteomics Group, Ontario Center for Structural Proteomics,University of Toronto, 4Center for Bioproducts and Bioenergy,Washington State University
Chapitres
- 00:05Titre
- 01:50Electroporation
- 02:46Fixation and Immunofluorescence Staining
- 04:21Affinity Purification
- 06:11Results: Electroporation of Salmonella Effector into HeLa Cells
- 07:50Conclusion
Electroporation was used to insert purified bacterial virulence effector proteins directly into living eukaryotic cells. Protein localization was monitored by confocal immunofluorescence microscopy. This method allows for studies on trafficking, function, and protein-protein interactions using active exogenous proteins, avoiding the need for heterologous expression in eukaryotic cells.
