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An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
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Chapitres
- 00:05Titre
- 01:44RNA Transcription with Corrole Treatment
- 03:09Agarose Gel Electrophoresis (1% Agarose Gel)
- 05:58RNA Quantification via UV-Vis Spectroscopy
- 06:47Results: Assessment of RNA Transcription by Agarose Gel Electrophoresis and UV-Vis Spectroscopy
- 08:17Conclusion
Gallium(III) 5,10,15-(tris)pentafluorophenylcorrole and its freebase analogue exhibit low micromolar cell cytotoxicity. This manuscript describes an RNA transcription reaction, imaging RNA with an ethidium bromide-stained gel, and quantifying RNA with UV-Vis spectroscopy, in order to assess transcription inhibition by corroles and demonstrates a straightforward method of evaluating anticancer candidate properties.
