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Bioengineering

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JoVE Journal Bioengineering
An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
 

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles

Article DOI: 10.3791/52355-v • 09:00 min • March 18th, 2015
March 18th, 2015 •
1, 1, 1, 2, 2, 1, 1
1Division of Chemistry and Chemical Engineering, California Institute of Technology, 2Department of Molecular Medicine, Beckman Research Institute of the City of Hope

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Summary
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Gallium(III) 5,10,15-(tris)pentafluorophenylcorrole and its freebase analogue exhibit low micromolar cell cytotoxicity. This manuscript describes an RNA transcription reaction, imaging RNA with an ethidium bromide-stained gel, and quantifying RNA with UV-Vis spectroscopy, in order to assess transcription inhibition by corroles and demonstrates a straightforward method of evaluating anticancer candidate properties.

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Transcription Inhibition RNA Transcription Assay Gallium(III) 5,10,15-(tris)pentafluorophenylcorrole 5,10,15-(tris)pentafluorophenylcorrole Actinomycin D Triptolide Anticancer Drug Mechanism Of Action
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