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A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl
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Journal JoVE Chimie
A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl

A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl

DOI:
10.3791/52419-v

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05:20 min

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November 28, 2014

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, ,
1Cancer Immunology Program,Peter MacCallum Cancer Centre

Chapitres

  • 00:05Titre
  • 01:00Sample Preparation
  • 02:07Granzyme B Activity Assay
  • 03:30Results: The Ala-Ala-Asp-S-Benzyl Substrate is Specific for Granzyme B
  • 04:53Conclusion

Summary

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Traduction automatique

We describe a simple, quantitative colorimetric assay that specifically measures the proteolytic activity of human, mouse or rat Granzyme B (GzmB). This protocol can be easily adapted for determining protease activity of other granule serine proteases by the hydrolysis of other synthetic peptide substrates with an appropriate recognition sequence.

Tags

Granzyme BColorimetric AssayProteolytic ActivityBoc-Ala-Ala-Asp-S-BzlCytotoxic T LymphocytesNatural Killer CellsSerine ProteaseApoptosisSubstrate SpecificityRecombinant Proteases

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