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JoVE Journal Neuroscience
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
 

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

Article DOI: 10.3791/52475-v • 08:45 min • February 19th, 2015
February 19th, 2015 •
1, 2, 2, 1,3
1Division of Brain Sciences, Department of Medicine, Imperial College London, 2Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, 3Department of Internal Medicine, Endocrinology, Yale University School of Medicine

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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of neurodegeneration.

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Keywords: Primary Mesencephalic Dopaminergic Neurons Embryonic Rodent Brains Parkinson's Disease Neurodegenerative Disorder Cell Culture Midbrain Dopaminergic Neurons E12.5 Mouse E14.5 Rat Neuronal Morphology Physiological Characteristics
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