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Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
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Journal JoVE Biologie
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

DOI:
10.3791/52484-v

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09:39 min

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March 17, 2015

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, ,
1Blood Systems Research Institute, 2Department of Medicine,University of California, San Francisco, 3Department of Laboratory Medicine,University of California, San Francisco

Chapitres

  • 00:05Titre
  • 01:23Individual Extracellular Vesicle Detection
  • 05:33Bead-based Detection of EVs and Exosomes
  • 07:19Results: Representative Individual and Bead-based Detection Data
  • 08:49Conclusion

Summary

Traduction automatique

Traduction automatique

Many different methods exist for the measurement of extracellular vesicles (EVs) using flow cytometry (FCM). Several aspects should be considered when determining the most appropriate method to use. Two protocols for measuring EVs are presented, using either individual detection or a bead-based approach.

Tags

Extracellular VesiclesFlow CytometryCell-cell CommunicationBiological ProcessesAnalysis TechniquesEV ProcessingBead-based ApproachIndividual Detection MethodSignal-to-noise RatioBackground FluorescenceClinical SamplesExosomes

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