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Простой основе полиакриламида многолуночные Жесткость Анализ по изучению жесткости зависимых клеточных ответов
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Simple Polyacrylamide-based Multiwell Stiffness Assay for the Study of Stiffness-dependent Cell Responses
DOI:

07:45 min

March 25, 2015

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Chapters

  • 00:05Title
  • 01:33Preparation of Hydrogel-associated Solutions and Aliquots
  • 02:41Hydrogel Preparation
  • 04:29Multiwell Plate Assembly, Collagen Coating, and Sterilization
  • 06:00Results: Polyacrylamide Gel Preparation in Multiwell Plates for Stiffness-dependent Cell Response Studies
  • 07:19Conclusion

Summary

Automatic Translation

Here, a method that enables quick, efficient, and inexpensive preparation of polyacrylamide gels in a multiwell plate format is described. The method does not require any specialized equipment and could be easily adopted by any research laboratory. It would be particularly useful in research focused on understanding stiffness-dependent cell responses.

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