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Bioengineering

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JoVE Journal Bioengineering
3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
 

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Article DOI: 10.3791/59226-v • 05:57 min • May 24th, 2019
May 24th, 2019 •
1, 2, 1, 2,3, 1
1Department of Biomedical Engineering and Yale Systems Biology Institute, Yale University, 2Department of Biomedical Engineering, Johns Hopkins University, 3Center for Cell Dynamics and Department of Cell Biology, Johns Hopkins University

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Summary
Automatic Translation

We describe a method to construct devices for 3D culture and experimentation with cells and multicellular organoids. This device allows analysis of cellular responses to soluble signals in 3D microenvironments with defined chemoattractant gradients. Organoids are better than single cells at detection of weak noisy inputs.

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Keywords: 3D Analysis Multi-cellular Response Chemoattractant Gradients 3D Tissue-like Environment PDMS Device Mesofluidic Device Stereo Lithography Vacuum Degassing PDMS Curing Cell Loading Collagen Cell Suspension Cell Culture 3D Physiological Environment
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