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一个<em>在体外</em>对雌激素敏感的乳腺癌休眠模式在骨髓中:分子机理研究与假设生成的一个工具
Journal JoVE
Médecine
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Journal JoVE Médecine
An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow: A Tool for Molecular Mechanism Studies and Hypothesis Generation
DOI:

08:48 min

June 30, 2015

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Chapitres

  • 00:05Titre
  • 01:36Clonogenic Assay Plate Preparation
  • 04:13Clonogenic Assay
  • 06:34Results: Pi3 Kinase Inhibitors Activate the Doormant Phenotype in Estrogen Sensitive Cells
  • 07:59Conclusion

Summary

Traduction automatique

We developed an in vitro model of dormancy in the bone marrow for estrogen-sensitive breast cancer cells. The goal of this protocol is to demonstrate use of the model for the study of the molecular and cellular biology of dormancy and for generation of hypotheses for subsequent testing in vivo.

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