/
/
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:09Mouse Lymph Node and Spleen Isolation
- 02:11Tissue Processing and CD4+ Enrichment
- 04:22Naïve CD4+ T Cell Sorting and In vitro Differentiation
- 05:49Results: Representative In vitro Th Differentiation Data
- 06:40Conclusion
Naïve CD4+ T cells polarize to various subsets depending on the environment at the time of activation. The differentiation of naïve CD4+ T cells to various effector subsets can be achieved in vitro through the addition of T cell receptor stimuli and specific cytokine signals.
