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Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
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Chapitres
- 00:05Titre
- 01:23Preparations
- 02:36Chariot Complex Formation
- 03:51Transfecting the Cells
- 05:41Imaging and Reconstruction
- 09:35Results: Antibody Interference in HEK293 Cells and Primary Hippocampal Neurons
- 11:08Conclusion
Controlling protein expression is not only essential to every organism alive, but also an important strategy to investigate protein functions in cellular models. The protocol presented shows the application of antibody interference in mammalian cells including primary hippocampal neurons and demonstrates the use of three-dimensional reconstructions in studying protein function.
