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Geração e Análise Quantitativa de Baixa Freqüência ultra-som pulsado determinar Sonic sensibilidade dos tratados e não tratados células neoplásicas
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells
DOI:

09:05 min

July 22, 2015

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Chapitres

  • 00:05Titre
  • 01:46Cell Sonifier System Cleaning, Setup, and Intensity/Function Assessment
  • 03:30Cell Sonication and Damage Assessment
  • 05:07XTT Assay Assessment of Cell Viability and Mitochondria Activity
  • 06:35Results: Effects of Different Levels of Amplitude on Ultrasonically Lysed U937 and THP1 Cells
  • 08:08Conclusion

Summary

Traduction automatique

Selective damage of human leukemia cells can be achieved through a novel approach of applying low frequency ultrasound both with and without chemotherapeutic pretreatment of leukemic and normal hematopoietic cells.

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