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A Novel Kültür Modeli İnsan Pluripotent için Plasenta klimalı Medyada Jelatin üzerinde hücre çoğalma Kök
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
DOI:

07:33 min

August 03, 2015

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Chapitres

  • 00:05Titre
  • 01:22Cell Isolation from Human Placenta
  • 03:47Harvesting Human Placenta-derived Cell Conditioned Medium
  • 05:18Culture of Human Embryonic Stem Cells and Induction of Pluripotent Stem Cells
  • 05:59Results: hPSC Culture in Human Placental-derived Cell Culture Medium Mimics that of the Gold Standard
  • 07:05Conclusion

Summary

Traduction automatique

This protocol provides a simple and efficient way to propagate human pluripotent stem cells (hPSCs) using only conditioned media derived from the human placenta in a gelatin-coated dish without additional exogenous supplementation or hPSC-specific synthetic substrata.

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