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JoVE Journal Developmental Biology
Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
 

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Article DOI: 10.3791/53280-v • 09:16 min • December 14th, 2015
December 14th, 2015 •
1,2, 1,2
1Department of Dermatology, UCSD Stem Cell Program, University of California, San Diego, 2Department of Cellular and Molecular Medicine, UCSD Stem Cell Program, University of California, San Diego

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résumé
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The goal of this paper is to provide a comprehensive and detailed protocol on how to generate genetically modified human organotypic skin from epidermal keratinocytes and devitalized human dermis.

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Keywords: Organotypic Skin Cultures Genetically Modified Keratinocytes Devitalized Human Dermis Epidermal Differentiation Stratification Retroviruses Gene Overexpression Gene Knockdown Skin Homeostasis Gene Expression Profiling Immunostaining Chromatin Immunoprecipitation High-throughput Sequencing
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