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Laser-guided Neuronal Tracing In Brain Explants
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Journal JoVE Neurosciences
Laser-guided Neuronal Tracing In Brain Explants

Laser-guided Neuronal Tracing In Brain Explants

DOI:
10.3791/53333-v

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06:40 min

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November 25, 2015

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,
1Department of Physiology and Biophysics,University of Colorado School of Medicine

Chapitres

  • 00:05Titre
  • 00:56Optical Genotyping
  • 02:25Preparing for the Tracer Injections
  • 03:05Anterograde/Retrograde Tracing
  • 05:01Results: Labeling Connections within the Trapezoid Body
  • 05:49Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.

Tags

Laser-guided Neuronal TracingBrain ExplantsIn Vitro Tracer InjectionElectroporationFluorescent Genetic MarkersBand-pass Filter GogglesNeurocircuit Functionality

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