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1Department of Mechanical Engineering,Virginia Polytechnic Institute and State University, 2School of Biomedical Engineering and Sciences,Virginia Polytechnic Institute and State University, 3Department of Biology,University of Maryland, 4College of Engineering,University of Georgia, 5Department of Engineering Sciences and Mechanics,Virginia Polytechnic Institute and State University
Chapitres
- 00:05Titre
- 00:48Preparation of Liposomes
- 02:00Manufacture of the Oil Reservoir and Preparation of Electrodes
- 04:06Setting Up the Experiment
- 06:22Formation of the Lipid Bilayer
- 07:49Results: Bilayer Formation and Activation of MscL Channels in Response to Harmonic Mechanical Stimuli
- 08:59Conclusion
Canais mechanosensitive bacterianas podem ser utilizados como transdutores mecanoelétrica em dispositivos biomoleculares. Bilayers de interface de gotículas (DIBs), de inspiração celulares blocos de construção para tais dispositivos, representam novas plataformas para incorporar e estimular canais mechanosensitive. Aqui, demonstramos um novo método baseado na micropipeta de formar DIB, permitindo que o estudo de canais mechanosensitive sob estimulação mecânica.
