This content is Free Access.
1Department of Mechanical Engineering,Virginia Polytechnic Institute and State University, 2School of Biomedical Engineering and Sciences,Virginia Polytechnic Institute and State University, 3Department of Biology,University of Maryland, 4College of Engineering,University of Georgia, 5Department of Engineering Sciences and Mechanics,Virginia Polytechnic Institute and State University
Chapitres
- 00:05Titre
- 00:48Preparation of Liposomes
- 02:00Manufacture of the Oil Reservoir and Preparation of Electrodes
- 04:06Setting Up the Experiment
- 06:22Formation of the Lipid Bilayer
- 07:49Results: Bilayer Formation and Activation of MscL Channels in Response to Harmonic Mechanical Stimuli
- 08:59Conclusion
Bakterielle mekanosensitive kanaler kan anvendes som mechanoelectrical transducere i biomolekylære enheder. Dråbe grænseflade dobbeltlag (DIBS), celle-inspirerede byggestenene til disse enheder, udgør nye platforme at indarbejde og stimulere mekanosensitive kanaler. Her viser vi en ny mikropipette-baseret fremgangsmåde til dannelse af DIBS, tillader studiet af mekanosensitive kanaler under mekanisk stimulering.
