Journal
/
Biologie
/
Generazione e Cultura Sangue escrescenza endoteliali cellule da sangue periferico umano
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
Please note that all translations are automatically generated. Click here for the English version.

Generazione e Cultura Sangue escrescenza endoteliali cellule da sangue periferico umano

DOI:
10.3791/53384-v

•

11:00 min

•

December 23, 2015

•

, , , , , ,
1Department of Medicine,University of Cambridge, 2Papworth Hospital

Chapitres

  • 00:05Titre
  • 00:45Density Gradient Centrifugation
  • 01:50Collagen Coating of T-75 Flask and Preparation of Culture Medium
  • 03:00Collection and Plating of Peripheral Blood Mononuclear Cells
  • 04:51Long-term Cell Culture
  • 06:58Freezing and Thawing BOEC Cultures
  • 08:44Results: Brightfield Images of BOEC Cultures and Immunofluorescent Staining of BOECs
  • 09:47Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol allows for the reliable generation and characterization of blood outgrowth endothelial cells (BOECs) from a small volume of adult peripheral blood. BOECs can be used as a surrogate for endothelial cells from patients with vascular disorders and as a substrate for the generation of induced pluripotent stem cells.

Tags

Keywords: Blood Outgrowth Endothelial CellsBOECsEndothelial ProgenitorsEndothelial DysfunctionVascular DisordersPrimary Endothelial CellsCell CulturePatient-derivedEndothelial Cell TransplantationInduced Pluripotent Stem CellsIPSCsNuclear ReprogrammingPeripheral Blood

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/53384?article_type=v

Arricchimento di cellule NK dal sangue umano con il kit RosetteSep Technologies StemCell
check_url/fr/53384?article_type=v

Isolamento e la coltura di cellule endoteliali polmonari da topi neonati
check_url/fr/53384?article_type=v

Caratterizzazione fenotipica e funzionale delle cellule endoteliali Colony Forming derivato dal sangue del cordone ombelicale umano
check_url/fr/53384?article_type=v

Generazione di cellule pluripotenti indotte umane staminali da sangue periferico mediante il STEMCCA lentivirale Vector
check_url/fr/53384?article_type=v

Purificazione e microRNA profiling di Exosomes derivati ​​dal sangue e cultura dei media
check_url/fr/53384?article_type=v

Isolamento dei vasi sanguigni derivati ​​da Multipotent precursori del muscolo scheletrico umano
check_url/fr/53384?article_type=v

Generazione efficiente delle cellule iPS da Blood Utilizzando episomi e HDAC inibitori
check_url/fr/53384?article_type=v

A due fotoni Imaging di Intracellulare Ca<sup> 2+</sup> Manipolazione e l'ossido nitrico Produzione in endoteliale e della muscolatura liscia cellule di un isolato Rat Aorta
check_url/fr/53384?article_type=v

Le proteine ​​immunofluorescenza di endogeni ed esogeni Centromere-cinetocoro
check_url/fr/53384?article_type=v

Isolamento e caratterizzazione di microvescicole da sangue periferico

Read Article