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Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

DOI:
10.3791/53459-v

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13:16 min

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December 22, 2015

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, , , , , , ,
1Skeletal Biotech Laboratory,The Hebrew University–Hadassah Faculty of Dental Medicine, 2Department of Surgery,Cedars-Sinai Medical Center, 3Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, 4Biomedical Imaging Research Institute,Cedars-Sinai Medical Center

Chapitres

  • 00:05Titre
  • 00:54Preparation of Bone Allografts
  • 02:38Calvarial Defect Surgery
  • 05:32Micro-computed Tomography for Characterization of the Vascular Tree
  • 10:50Results: Cranial Bone Autograft and Allograft Analysis
  • 12:32Conclusion

Summary

Traduction automatique

Traduction automatique

Implantation of autologous and allogeneic bone grafts constitute accepted approaches to treat major craniofacial bone loss. Yet the effect of graft composition on the interplay among neovascularization, cell differentiation and bone formation is unclear. We present a multimodal imaging protocol aimed to elucidate the angiogenesis-osteogenesis interdependence at the graft proximity.

Tags

Computed TomographyOptical ImagingOsteogenesis-angiogenesis CouplingBone AutograftsBone AllograftsVascularizationMicro-CTFluorescence ImagingBioluminescence ImagingBone RegenerationStem Cell RecruitmentArteriole FormationBone Mineralization

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