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具体的基因组区域及相关分子鉴定enChIP隔离
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP
DOI:

09:26 min

January 20, 2016

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Chapitres

  • 00:05Titre
  • 00:41Preparation of Tagged Chromatin
  • 04:16Chromatin Immunoprecipitation
  • 06:41Immunoprecipitated Chromatin Analysis
  • 08:08Results: enChIP to Telomere and the Interferon Regulatory Factor-1 (IRF-1) Promoter Regions
  • 09:00Conclusion

Summary

Traduction automatique

The identification of molecules associated with specific genomic regions of interest is required to understand the mechanisms of regulation of the functions of these regions. This protocol describes procedures to perform engineered DNA-binding molecule-mediated chromatin imunoprecipitation (enChIP) for identification of proteins and RNAs associated with a specific genomic region.

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