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RNAi-Trigger Lieferung in<em> Anopheles gambiae</em> Pupae
Journal JoVE
Biologie
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Journal JoVE Biologie
RNAi Trigger Delivery into Anopheles gambiae Pupae
DOI:

09:58 min

March 08, 2016

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Chapitres

  • 00:05Titre
  • 00:57Synthesis and Preparation of Double Stranded RNA (dsRNA)
  • 02:10Preparations for dsRNA Injections
  • 04:07Microinjecting Pupae with dsRNA
  • 06:22Culturing and Assessing Injected Pupae
  • 07:12Results: Injection Survivorship and SRPN2 Knockdown by dsRNA Injection
  • 08:34Conclusion

Summary

Traduction automatique

RNA interference (RNAi) is an extremely valuable tool for uncovering gene function. However, the ability to target genes using RNAi during pre-adult stages is limited in the major human malaria vector Anopheles gambiae. We describe an RNAi protocol to reduce gene function via direct injection during pupal development.

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