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Developmental Biology

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JoVE Journal Developmental Biology
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
 

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Article DOI: 10.3791/53754-v • 09:04 min • January 30th, 2017
January 30th, 2017 •
*1, *1, 2, 1,2, 3, 1
1Department of Agricultural, Food and Nutritional Science, University of Alberta, 2Livestock Research Branch, Alberta Agriculture and Forestry, 3Laboratory of Functional Genomics of Early Embryonic Development, Université Laval
* These authors contributed equally

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Microarray technology allows quantitative measurement and gene expression profiling of transcripts on a genome-wide basis. Therefore, this protocol provides an optimized technical procedure in a two-color custom made bovine array using Day 7 bovine embryos to demonstrate the feasibility of using low amount of total RNA.

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Keywords: Transcriptome Profiling Bovine Pre-implantation Embryos Two-color Microarray RNA Extraction Column-based Purification Pico-scale RNA Lysis Buffer Conditioning Buffer Ethanol Wash Buffers DNase Treatment Elution Buffer
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