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Chapitres
- 00:05Titre
- 00:45Preparation of RNA-Seq Samples
- 02:47Preparation of ChIP-Seq Samples
- 06:13Metabolomics Assay Sample Collection and Cell Count for Normalization
- 07:32Results: Metabolic Changes in MCF7 Cells from Estradiol Treatment
- 09:15Conclusion
Integration of data from genome-wide sequencing experiments and metabolomics experiments is a challenge. In this paper we report, for the first time, generation, analysis and integration of transcriptome, cistrome and metabolome data from breast cancer cells treated with estradiol.
Tags
Keywords:
Systems BiologyMetabolic RegulationEstrogen Receptor SignalingBreast CancerTranscriptomeSystromeMetabolomeTranscription FactorsMetabolismCell AggressivenessMCF7 CellsRNA IsolationChloroformIsopropanolEthanolDEPC WaterFormaldehydeChromatin Cross-linkingCell Lysis BufferChromatin Fragmentation
