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BMP rakip GREM2 kullanma Pluripotent Kök Hücreleri Atriyal kardiyomiyositlerde Farklılaşma
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2
DOI:

08:52 min

March 10, 2016

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Chapitres

  • 00:05Titre
  • 00:33Murine Embryonic Stem Cell (mESC) Culture and Passaging
  • 03:16Embryoid Body (EB) Preparation and Grem2 Treatment
  • 06:12Results: Grem2-induction of Cardiomyocyte Differentiation
  • 08:00Conclusion

Summary

Traduction automatique

Generating cardiomyocytes from pluripotent stem cells in vitro allows access to large amounts of cardiac tissue in vitro for basic science and clinical applications. This protocol uses the atrializing factor Grem2 to both increase the numbers of cardiomyocytes obtained and to generate cardiomyocytes with an atrial phenotype.

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