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初期の飢餓応答でCoronin Aの機能を調査<em>細胞性粘菌</em>凝集アッセイによって
Journal JoVE
Biologie
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Journal JoVE Biologie
Investigating the Function of Coronin A in the Early Starvation Response of Dictyostelium discoideum by Aggregation Assays
DOI:

07:48 min

June 18, 2016

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Chapitres

  • 00:05Titre
  • 00:58Observe the Early Starvation Response of Dictyostelium discoideum by Time-lapse Microscopy
  • 02:14cAMP Pulsing of Dictyostelium discoideum Cells During Starvation
  • 03:54Induction of Dictyostelium discoideum Development Through Exposure to Conditioned Medium
  • 05:32Results: cAMP Pulses Rescue the Early Developmental Defect of corA- Dictyostelium discoideum
  • 06:45Conclusion

Summary

Traduction automatique

The social amoeba Dictyostelium discoideum undergoes a developmental transition into a multicellular organism when starved. The evolutionary conserved protein coronin A plays a crucial role in the initiation of development. Using aggregation assays as our main method, we aim to elucidate the role of coronin A in early development.

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