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Die Erzeugung von Microtumors Mit 3D Human Biogel Kultursystem und Patienten stammGlioblastomZellen für Kinomic Profilieren and Drug Response-Testing
Journal JoVE
Médecine
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Journal JoVE Médecine
Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing
DOI:

09:24 min

June 09, 2016

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Chapitres

  • 00:05Titre
  • 01:31Tumor Disaggregation
  • 04:05Microtumor Generation and Drug Dosing
  • 05:54Microtumor Morphologic and Phenotypic Analyses
  • 07:08Results: Representative Microtumor Growth and Analyses
  • 08:38Conclusion

Summary

Traduction automatique

Patient-derived xenografts of glioblastoma multiforme can be miniaturized into living microtumors using 3D human biogel culture system. This in vivo-like 3D tumor assay is suitable for drug response testing and molecular profiling, including kinomic analysis.

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