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JoVE Journal Bioengineering
Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity
 

Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity

Article DOI: 10.3791/54148-v • 08:54 min • July 25th, 2016
July 25th, 2016 •
1, 2, 1
1Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, 2Mucosal Immunology and Biology Research Center, Massachusetts General Hospital for Children

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Cells growing in a three-dimensional (3-D) environment represent a marked improvement over cell cultivation in 2-D environments (e.g., flasks or dishes). Here we describe the development of a multicellular 3-D organotypic model of the human intestinal mucosa cultured under microgravity provided by rotating-wall-vessel (RWV) bioreactors.

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Keywords: 3D Organotypic Model Human Intestinal Mucosa Microgravity Rotating Wall Vessel Bioreactors Endothelial Cells Fibroblasts Collagen Laminin Fibronectin Heparin Sulfate Proteoglycan Cell Culture Tissue Engineering
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