Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 00:43TAE-SDS Agarose Gel Preparation
- 02:02Sample Loading and Electrophoresis Separation
- 03:32Agarose Gel Reduction
- 04:08Vacuum Blotter Assembly and Sample Transfer to a Nitrocellulose Membrane
- 06:33Membrane Blocking and Detection
- 08:08Results: Enhanced Analysis of High-molecular-weight Glycoproteins
- 08:45Conclusion
Mucins are high-molecular-weight glycoconjugates, with size ranging from 0.2 to 200 megadalton (MDa). As a result of their size, mucins do not penetrate conventional polyacrylamide gels and require larger pores for separation. We provide a detailed protocol for mucin agarose gel electrophoresis to assess relative quantification and study polymer assembly.
