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Isolamento di perivascolare Multipotent precursori delle cellule popolazioni dal cardiache umane Tissue
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1Centre for Cardiovascular Science, Queen’s Medical Research Institute,University of Edinburgh, 2Department of Bioengineering and Orthopaedic Surgery,University of Pittsburgh, 3Research Laboratory of Electronics and Department of Biological Engineering,Massachusetts Institute of Technology, 4MRC Centre for Regenerative Medicine,University of Edinburgh, 5Stem Cell Research Center, Department of Orthopaedic Surgery,University of Pittsburgh, 6Department of Orthopaedic Surgery,University of Texas Health Science Center at Houston, 7Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine,University of California at Los Angeles
Chapitres
- 00:05Titre
- 01:06Tissue Digestion and Cell Isolation
- 03:27Cell Labelling and Sorting
- 06:05Cell Culture
- 06:54Results: FAS Purification of Myocardial Perivascular Cells and Perivascular Cell Morphology
- 07:39Conclusion
tessuto cardiaco umano alberga popolazioni di cellule precursore perivascolari multipotenti che possono essere adatti per la rigenerazione del miocardio. La tecnica qui descritta permette l'isolamento e la purificazione simultanea di due popolazioni di cellule stromali multipotenti associati con i vasi sanguigni nativi, cioè CD146 + CD34 - periciti e CD34 + CD146 - cellule avventiziale, dal miocardio umano.
