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Yetişkin Rats gelen bozulmamış Dorsal Kök Ganglia üzerinde Patch Kelepçe Kayıtlar
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats
DOI:

12:00 min

September 29, 2016

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Chapitres

  • 00:05Titre
  • 00:55Dorsal Root Ganglia Dissection
  • 04:09Dorsal Root Ganglia Digestion
  • 06:11Patch Clamp Recordings
  • 10:05Results: Measurement of Neuronal Excitability and Ligand-induced Currents in Small DRG Neurons
  • 10:59Conclusion

Summary

Traduction automatique

This manuscript describes how to prepare intact dorsal root ganglia for patch clamp recordings. This preparation maintains the microenvironment for neurons and satellite glial cells, thus avoiding the phenotypic and functional changes seen using dissociated DRG neurons.

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