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JoVE Journal Genetics
Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
 

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Article DOI: 10.3791/54446-v • 06:03 min • September 20th, 2016
September 20th, 2016 •
*1, *2, 3
1Super-Bacteria Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 2Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Bioengineering (KRIBB), 3Department of Biotechnology, The Catholic University of Korea
* These authors contributed equally

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résumé
Automatic Translation

Here, we present a protocol to obtain adaptive laboratory evolution of microorganisms under conditions using chemostat culture. Also, genomic analysis of the evolved strain is discussed.

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Adaptive Laboratory Evolution Microorganisms Chemostat Evolution Microbiology Adrenal Function Relationships Stress Responses Metabolic Engineering Laboratory Conditions Continuous Selection Equipment Preparation Initial Medium Stress Medium High Stress Medium Inoculate E.coli Test Tube Incubate Shaking Incubator Chemostat Jar Aeration Agitation Silicon Tubing Pumps Pre-culture Outlet Pump Exponential Phase Optical Density At 600 Nanometers
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