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Chapitres
- 00:05Titre
- 01:16Creating Plasmids as Template Standards
- 02:20Analyzing Primer Specificity for Absolute qPCR
- 05:49Performing Relative qPCR Assays
- 06:57Analyzing Absolute qPCR Data for Unknown Samples
- 08:27Results: Measurement of STAT3 Splice Variant Levels in Eosinophils Treated with Cytokines
- 10:06Conclusion
Tandem splicing events occur at sites less than 12 nucleotides apart. Quantifying ratios of such splice variants is feasible using an absolute quantitative PCR approach. This manuscript describes how splice variants of the gene STAT3, in which two splicing events results in Serine-701 inclusion/exclusion and α/β C-termini, can be quantified.
