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백혈구가 풍부한 혈액 샘플의 할로겐 퍼 옥시 다제 활동의 신속하고 구체적인 평가
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
DOI:

05:17 min

July 28, 2016

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Chapitres

  • 00:05Titre
  • 00:36Hypotonic Erythrocyte Lysis
  • 02:20Halogenating Peroxidase Activity Staining
  • 03:31Results: Leukocyte Enrichment and APF-derived Fluorescence in a Rat Blood Micro-sample
  • 04:29Conclusion

Summary

Traduction automatique

This protocol describes the quick enrichment of leukocytes from small blood samples for a subsequent specific determination of the halogenating peroxidase activity within the cells. The method can be applied to human and non-human material and may contribute to the evaluation of new inflammatory markers.

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