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核酸的聚苯胺传感器
Journal JoVE
Biochimie
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Journal JoVE Biochimie
A Polyaniline-based Sensor of Nucleic Acids
DOI:

07:58 min

November 01, 2016

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Chapitres

  • 00:05Titre
  • 00:55Processable PANI Synthesis
  • 02:40PANI-probe Mixing and UV Irradiation and Hybridization of PANI-probe
  • 04:21Emission Steady State Fluorescence Measurement and Fluorescence Microscopy Measurement of Hybridized Duplex
  • 05:45Results: Use of PANI Fluorescence to Detect Nucleic Acid Hybridization
  • 06:53Conclusion

Summary

Traduction automatique

Nucleic acids are common analytes for assessing biological systems; however, bias from enzymatic manipulation can cause concern. Here a method is described for label-free detection of nucleic acids using polyaniline. This sensitive, cost-effective sensor technology can distinguish single nucleotide differences between molecules.

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