Journal
/
Chimie
/
Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
Journal JoVE
Chimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Chimie
Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

DOI:
10.3791/54611-v

•

10:13 min

•

November 12, 2016

•

, ,
1Center for Applied Plant Sciences,The Ohio State University, OARDC, 2Department of Plant Pathology,The Ohio State University, OARDC, 3Department of Horticulture and Crop Science,The Ohio State University, OARDC, 4OARDC Metabolite Analysis Cluster,The Ohio State University, OARDC

Chapitres

  • 00:05Titre
  • 00:49Sample Preparation
  • 02:30Organic Acid Standard Preparation
  • 04:34Organic Acid Extraction
  • 06:35SCCA Detection Run Execition and Data Collection
  • 08:18Results: Quantification of Organic Acid Levels in Green Coffee Beans
  • 09:35Conclusion

Summary

Traduction automatique

Traduction automatique

This article presents a method for the detection and quantification of organic acids from plant material using free zonal capillary electrophoresis. An example of the potential application of this method, determining the effects of a secondary fermentation on organic acid levels in coffee seeds, is provided.

Tags

Capillary ElectrophoresisOrganic AcidsCoffea ArabicaCoffee SeedsShort Chain Carboxylic Acids (SCCAs)Sample PreparationLiquid Nitrogen GrindingAuthentic StandardsExternal And Internal StandardsCitric AcidMalic AcidAcetic AcidLactic AcidAdipic Acid

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/54611?article_type=v

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
check_url/fr/54611?article_type=v

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
check_url/fr/54611?article_type=v

The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
check_url/fr/54611?article_type=v

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
check_url/fr/54611?article_type=v

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
check_url/fr/54611?article_type=v

Grafting Multiwalled Carbon Nanotubes with Polystyrene to Enable Self-Assembly and Anisotropic Patchiness
check_url/fr/54611?article_type=v

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds
check_url/fr/54611?article_type=v

Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos
check_url/fr/54611?article_type=v

Microfluidic Preparation of Liquid Crystalline Elastomer Actuators
check_url/fr/54611?article_type=v

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Read Article