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長期用水中フィルターペーパーサンドイッチ<em>例OVO</emニワトリ初期胚の>タイムラプスイメージング
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos
DOI:

07:29 min

December 28, 2016

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Chapitres

  • 00:05Titre
  • 00:37Submerged Filter Paper Sandwich Production
  • 05:11Results: Time-lapse Acquisition of Chick Embryonic Development
  • 06:48Conclusion

Summary

Traduction automatique

Here, we present a new technique to culture chick embryos ex ovo for time-lapse imaging using transmitted light and fluorescence microscopy. The submerged filter paper sandwich is a variant of the well-established filter paper carrier technique1 that allows for the culturing of chick embryos fully-submerged in a liquid culture medium.

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