/

/

制备及<em>在体内</em>使用基于活动的探针对<em>ñ</em> -acylethanolamine酸酰胺酶

Journal JoVE
Biochimie

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Biochimie

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Please note that all translations are automatically generated. Click here for the English version.

制备及<em>在体内</em>使用基于活动的探针对<em>ñ</em> -acylethanolamine酸酰胺酶

DOI:

10.3791/54652-v

•

11:01 min

•

November 23, 2016

•

Elisa Romeo, Silvia Pontis, Stefano Ponzano, Fabiola Bonezzi, Marco Migliore, Simona Di Martino, Maria Summa, Daniele Piomelli²

¹Drug Discovery and Development,Istituto Italiano di Tecnologia, ²Departments of Anatomy and Neurobiology, Pharmacology, and Biological Chemistry,University of California, Irvine School of Medicine

Chapitres

00:05Titre
01:11Preparation of 2-pyridyl Undec-10-ynyl Carbonate and Undec-10-ynyl 2-oxopyridine 1-carboxylate
02:44Preparation of Undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate
04:17Click Chemistry with Azide-PEG3-Biotin
05:14Removal of Excess Click Chemistry Reagents
06:29Streptavidin Enrichment
07:57Click Chemistry with Azide-PEG3-Fluor 545 on Tissue Slices
08:59Results: Synthesis and Applications of NAAA Activity Based Probe, ARN14686
10:21Conclusion

Summary

Traduction automatique

这里，我们描述一个基于活动的探针的制备和使用（ARN14686，十一碳-10- ynyl- -N - [（3 S）-2-代氮杂环丁烷-3-基]氨基甲酸），允许的检测和定量促炎酶ñ-acylethanolamine酸酰胺酶的活性形式（NAAA）， 在体外和离体。

Tags

Keywords: Activity-based Probe N-acylethanolamine Acid Amidase (NAAA)Synthesis In Vivo Use Fluorescence Microscopy Inflammatory Diseases Neurodegenerative Diseases Undecynenol DMAP DPC Dichloromethane Sodium Bicarbonate Sodium Sulfate Oxo Asetydenol Ammonium Asetate Diazapropalethylamine

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

对于高度纯化RNA的种子定量转录组分析的隔离中使用的有效方法

RNA 结合区质量谱鉴定的样品制备

鸡羽毛角蛋白水解物的制备及其在化妆品中的应用

用于筛查绝经后心血管功能障碍的外源雌激素治疗的活体雌激素缺乏小鼠模型

探头电喷电镀质谱的样品制备

使用核酸探针进行核酸活性的动力学筛选

优质发酵鱼产品的制备

使用微尺度热泳测量蛋白质-脂质相互作用

使用 HPLC-MS/MS 进行核苷/核苷酸含量测量的工厂样品制备

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Read Article