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Bioengineering

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JoVE Journal Bioengineering
Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells
 

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

Article DOI: 10.3791/54785-v • 10:23 min • December 13th, 2016
December 13th, 2016 •
1, 2, 3,4, 1, 5, 6
1Bristol Centre for Functional Nanomaterials, University of Bristol, 2Department of Materials, Imperial College London, 3Self Assembly Group, CIC nanoGUNE, 4Ikebasque, Basque Foundation for Science, 5School of Cellular and Molecular Medicine, University of Bristol, 6H.H. Wills Physics Laboratory, University of Bristol

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A protocol for the synthesis and cationization of cobalt-doped magnetoferritin is presented, as well as a method to rapidly magnetize stem cells with cationized magnetoferritin.

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Synthesis Cationized Magnetoferritin Ultra-fast Magnetization Cells Magnetic Nanoparticle Protein Cage Functionalize Rapid Attachment Magnetic Labeling MRI Magnetic Cell Separation Low Nanoparticle Concentrations Short Incubation Times Adverse Effects Deoxygenate Water Nitrogen Gas Cylinder Cling Film Nitrogen Gas Water Bath Double-jacketed Reaction Vessel HEPES Buffer PH 8.6 Deoxygenate Buffer Solution Magnetic Stirrer Apoferritin
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