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Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

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Biologie
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Journal JoVE Biologie

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

DOI:

10.3791/54874-v

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13:35 min

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May 05, 2017

•

Corinne Blancard, Bénédicte Salin

¹Institut de Biochimie et Génétique Cellulaires, Centre National de la Recherche Scientifique, UMR 5095,Université de Bordeaux

Chapitres

00:00Titre
01:38Preparation of Formvar Grid Film
03:53Preparation of Freeze-substitution Medium
05:06Preparation of Cells
06:11Liquefaction of Propane
07:32Plunge Freezing of Samples
08:32Freeze-substitution
09:04Sample Warming
10:57Results: Plunge Freezing Results in Improved Preservation of the Intracellular Structures
12:50Conclusion

Summary

Traduction automatique

This manuscript describes an easy-to-use and low-cost cryofixation method for visualizing suspension cells by transmission electron microscopy.

Tags

Plunge Freezing Ultrastructural Studies Immunolocalization Suspension Cells Transmission Electron Microscopy Freeze Substitution Chemical Fixation Cryofixation Formvar Film Glass Slide

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