Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 00:51Construction of the PUF RNA-binding Domain Scaffold with Customized RNA Binding Specificity by Overlapping PCR
- 02:05Transfection of Engineered Splicing Factors (ESFs) for Modulation of Exon Inclusion and Alternative Splice-site Use
- 03:43Cell Harvest and Identification of Spliced Isoforms
- 06:24Modulation of Endogenous Bcl-x Splicing and Apoptosis by ESFs
- 08:23Results: Subcellular Localization of ESFs in Transfected HeLa Cells
- 09:30Conclusion
