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Two Techniques to Create Hypoparathyroid Mice
 

Two Techniques to Create Hypoparathyroid Mice: Parathyroidectomy Using GFP Glands and Diphtheria-Toxin-Mediated Parathyroid Ablation

Article DOI: 10.3791/55010-v • 07:13 min • March 14th, 2017
March 14th, 2017 •
1,2, 2,3, 2,4, 2,4, 1
1Endocrine Unit, Massachusetts General Hospital, Harvard Medical School, 2West China School of Stomatology, Sichuan University, 3Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, 4State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University

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Mice with acquired hypoparathyroidism would be useful for studying novel drug therapies for hypoparathyroidism. Two procedures to create such mice are demonstrated. The GFP-PTX mouse is generated by surgical parathyroidectomy guided by green fluorescing parathyroid glands. A second, non-surgical approach is based on parathyroid-specific expression of the diphtheria toxin receptor.

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Keywords: Parathyroidectomy GFP Diphtheria Toxin Parathyroid Ablation Hypoparathyroidism Mouse Model Thyroid Function PTH Analog Therapy Bone Strength
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