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Cancer Research

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JoVE Journal Cancer Research
Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers
 

Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers

Article DOI: 10.3791/55027-v • 06:02 min • February 20th, 2017
February 20th, 2017 •
1, 1, 1
1Department of Medical Genetics, Signal Transduction Research Group, Faculty of Medicine and Dentistry, University of Alberta

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In this paper, we report a protocol describing an in vivo method to measure microtubule dynamic instability in docetaxel-resistant breast cancer cells (MCF-7TXT). In this method, a deconvolution microscopy imaging system is used to detect the expression of GFP-tubulin in target cells.

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Keywords: Microtubule Dynamic Instability Taxane-resistant Breast Cancer Live Cell Imaging GFP-tagged Alpha Tubulin Docetaxel Cell Biology Cell Culture Microscopy
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