Journal
/
Immunologie et infection
/
Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis

Use of a Monocyte Monolayer Assay to Evaluate Fcγ Receptor-mediated Phagocytosis

DOI:
10.3791/55039-v

•

06:27 min

•

January 02, 2017

•

,
1Department of Laboratory Medicine and Pathobiology,University of Toronto, 2Centre for Innovation,Canadian Blood Services

Chapitres

  • 00:05Titre
  • 00:58Peripheral Blood Mononuclear Cell (PBMC) Isolation
  • 02:22R2R2 Red Blood Cell Opsonization, Fc Receptor-mediated Phagocytosis, and Phagocytosis Quantification
  • 04:16Results: Representative Monocyte Phagocytosis Analyses
  • 05:28Conclusion

Summary

Traduction automatique

Traduction automatique

The monocyte monolayer assay (MMA) is an in vitro assay that utilizes isolated primary monocytes obtained from mammalian peripheral whole blood to evaluate Fcγ receptor (FcγR)-mediated phagocytosis.

Tags

Monocyte Monolayer AssayFc Receptor-mediated PhagocytosisImmunohematologyBlood TransfusionAuto-antibodiesAllo-antibodiesRed Blood CellsOpsonizationPolyclonal Anti-D AntibodiesPBMCDensity Gradient CentrifugationIn Vitro Functional Assay

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/55039?article_type=v

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
check_url/fr/55039?article_type=v

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
check_url/fr/55039?article_type=v

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
check_url/fr/55039?article_type=v

Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood
check_url/fr/55039?article_type=v

Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds
check_url/fr/55039?article_type=v

A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis
check_url/fr/55039?article_type=v

Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos
check_url/fr/55039?article_type=v

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
check_url/fr/55039?article_type=v

Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets
check_url/fr/55039?article_type=v

Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to Plasmodium falciparum Parasites by a Flow Cytometry-Based Assay

Read Article