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Biochemistry

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JoVE Journal Biochemistry
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
 

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Article DOI: 10.3791/55070-v • 08:09 min • January 7th, 2017
January 7th, 2017 •
1, 1
12bind GmbH

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MicroScale Thermophoresis (MST) is a sensitive technology to characterize aptamer-target interactions. This manuscript describes an MST protocol to characterize aptamer-small molecule interactions.

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Keywords: Aptamer ATP Microscale Thermophoresis Binding Site Mapping Cy5 Labeled DNA Aptamer Ligand Serial Dilution Fluorescence Signal Capillary Scan Molecular Interactions
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