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Bioengineering

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JoVE Journal Bioengineering
Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
 

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Article DOI: 10.3791/55101-v • 10:53 min • January 3rd, 2017
January 3rd, 2017 •
1, 2, 1,3
1Department of Biomedical Engineering, University of Delaware, 2Department of Electrical and Computer Engineering, University of Houston, 3Delaware Biotechnology Institute

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This protocol outlines the implementation of image-guided, laser-based hydrogel degradation to fabricate vascular-derived, biomimetic microfluidic networks embedded in poly(ethylene glycol) diacrylate (PEGDA) hydrogels. These biomimetic microfluidic systems may be useful for tissue engineering applications, generation of in vitro disease models, and fabrication of advanced "on-a-chip" devices.

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Keywords: Image-guided Laser-based Fabrication Vascular-derived Microfluidic Networks Confocal Microscope Femtosecond Pulse Laser Polyethylene Glycol Diacrylate Hydrogels Laser-based Degradation Biomimetic Microfluidic Networks Tissue Engineering Disease Models On-chip Devices Photopolymerization PEGDA Eosin Y NVP PDMS Mold
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