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A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains
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1Center for Metabolic and Degenerative Diseases, The Brown Foundation Institute of Molecular Medicine, Programs in Human and Molecular Genetics and Neuroscience, The Graduate School of Biomedical Sciences at Houston,The University of Texas Health Science Center at Houston, 2Department of Natural Sciences,University of Houston – Downtown, 3Department of Neurobiology and Anatomy, McGovern Medical School,The University of Texas Health Science Center at Houston
Chapitres
- 00:05Titre
- 01:12Microsurgical Dissection of Adult Fly Heads
- 02:56Immunofluorescent Staining of the Brains
- 04:44Mounting and Imaging of Brain Tissue
- 06:02Results: Anti-TH Staining in Dissected Fly Brains Allows Quantification of DA Neurons
- 07:40Conclusion
The adult Drosophila brain is a valuable system for studying neuronal circuitry, higher brain functions, and complex disorders. An efficient method to dissect whole brain tissue from the small fly head will facilitate brain-based studies. Here we describe a simple, one-step dissection protocol of adult brains with well-preserved morphology.
