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Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function
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Biologie
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Journal JoVE Biologie
Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

DOI:
10.3791/55201-v

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06:14 min

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June 11, 2017

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, , , ,
1Department of Surgery,Baltimore Veterans Affairs Medical Center, 2Department of Surgery,University of Maryland School of Medicine, 3Department of Physiology,University of Maryland School of Medicine, 4Center for Vascular and Inflammatory Diseases,University of Maryland School of Medicine

Chapitres

  • 00:05Titre
  • 00:49Murine Aortic Crush Injury and Post-procedure Care
  • 02:34Tissue Procurement
  • 03:54Results: Representative Effects of Crush Injury on Aortic Wall Thickening and Function
  • 05:37Conclusion

Summary

Traduction automatique

Traduction automatique

Restenosis following cardiovascular procedures (bypass surgery, angioplasty, or stenting) is a significant problem reducing the durability of these procedures. An ideal therapy would inhibit smooth muscle cell (VSMC) proliferation while promoting regeneration of the endothelium. We describe a model for simultaneous assessment of VSMC proliferation and endothelial function in vivo.

Tags

Murine Aortic Crush InjuryVascular Smooth Muscle Cell ProliferationReendothelializationRestenosisVascular BiologyIn Vivo ModelC57BL/6 MouseAnesthesiaLaparotomyAorta CrushEvans Blue DyeEndothelial Function

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