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Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes
Journal JoVE
Neurosciences
Author Produced
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Journal JoVE Neurosciences
Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes
DOI:

12:45 min

August 12, 2017

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Chapitres

  • 00:00Titre
  • 00:09Outline of Technique
  • 02:37Materials Needed and Surgical Preparations
  • 05:18Surgical Procedures: Trans-sclera Subretinal Injection
  • 10:12Résultats
  • 11:39Conclusion

Summary

Traduction automatique

Subretinal injection has been widely applied in preclinical studies of stem cell replacement therapy for age-related macular degeneration. In this visualized article, we describe a less risky, reproducible and precisely modified subretinal injection technique via the trans-scleral approach to deliver cells into rat eyes.

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