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Immunologie et infection
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Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry
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Immunologie et infection
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Journal JoVE Immunologie et infection
Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry

Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry

DOI:
10.3791/55222-v

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11:02 min

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January 20, 2017

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, , , , , ,
1Immunology and Allergy Unit, Department of Medicine Solna,Karolinska Institutet, 2Division of Medicine, Department of Public Health and Clinical Medicine,Umeå University, 3Divison of Infectious Diseases, Department of Clinical Microbiology,Umeå University

Chapitres

  • 00:05Titre
  • 01:01Glycol Methylacrylate (GMA) Resin Endobronchial Biopsy Embedment
  • 02:32GMA-embedded Endobronchial Biopsy Immunohistochemical Staining
  • 05:23Endobronchial Biopsy Enzymatic Digestion and Flow Cytometric Analysis
  • 08:17Results: Identifying Immune and Dendritic Cell Subsets in Endobronchial Biopsies
  • 10:08Conclusion

Summary

Traduction automatique

Traduction automatique

Lung-resident immune cells, including dendritic cells (DCs) in humans, are critical for defense against inhaled pathogens and allergens. However, due to the scarcity of human lung tissue, studies are limited. This work presents protocols to process human mucosal endobronchial biopsies for studying lung DCs using immunohistochemistry and flow cytometry.

Tags

Lung Dendritic CellsEndobronchial BiopsiesImmunohistochemistryFlow CytometryMucosal ImmunologyCOPDSarcoidosisLung CancerSpatial DistributionPhenotypic Identification

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