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Chapters
- 00:05Title
- 01:02Primer Design for PCR Amplification of Gene Flanks
- 02:49Production of OSCAR Constructs
- 05:48Agrobacterium tumefaciens Mediated Transformation (ATMT) of Fungi
- 07:45Deletion Mutant Identification by PCR
- 08:53Results: The OSCAR Method Generates a Gene Deletion Plasmid for Fungi in a Single Reaction
- 10:23Conclusion
Gendeletionsmutanter frembragt ved homolog rekombination er guldstandarden for genfunktionsstudier. OSCAR (One Step Construction of Agrobacterium-Recombination-ready-plasmids) fremgangsmåden til hurtig generering af deletionskonstruktioner er beskrevet. Agrobacterium- medieret svamptransformation følger. Endelig præsenteres en PCR-baseret bekræftelsesmetode for gendeletioner i svamptransformanter.
